This beginner-level educational PDF introduces the core principles of semen examination according to the WHO 5th and 6th editions. It is designed for undergraduate students, new embryology trainees, IVF laboratory observers and allied-health learners. The resource explains sample collection, macroscopic examination, sperm concentration, motility, morphology, vitality and important changes introduced in the sixth edition.
| Resource detail | PDF 1 | PDF 2 |
|---|---|---|
| Resource type | Educational PDF | Educational PDF |
| Learning level | Beginner | Beginner |
| Page count | 16 pages | 18 pages |
| File format | ||
| Access | Downloadable | Downloadable |
| Author | Manoj Kumar K | Manoj Kumar K |
| Publisher | Inside Embryo by Aurion | Inside Embryo by Aurion |
| Major category | ART Standards, Laws and Ethics | ART Standards, Laws and Ethics |
| Subcategory | ART Standards and Guidelines | ART Standards and Guidelines |
| Publication date | 3 August 2026 | 3 August 2026 |
What You Will Learn
- Understand the purpose of semen examination in male fertility assessment and assisted reproductive technology.
- Identify the main semen parameters, including volume, sperm concentration, total sperm number, motility, vitality and morphology.
- Describe the correct steps for semen sample preparation, collection, handling and laboratory examination.
- Compare the major similarities and differences between the WHO 5th and 6th editions.
- Interpret WHO lower reference values correctly without treating them as strict fertile–infertile cut-offs.
- Recognise common pre-analytical errors, quality-control requirements and reporting principles that influence semen-analysis results.



Educational Disclaimer: This PDF is provided for educational and professional-development purposes. It does not replace official manuals, institutional protocols, validated laboratory procedures, professional training, clinical consultation or clinical judgement. Readers should verify important information against the most recent authoritative guidance.
© Inside Embryo by Aurion. This PDF may be downloaded for personal learning and classroom reference. Republishing, modifying, reselling, removing the branding or commercially distributing the document without written permission is prohibited.


